As mentioned you want to get this measurement as close as possible. You do have a little bit of fudge factor in the adjustment of the bridge (what say a 1/8-1/4" depending on the bridge).
Most people try to get that measurement in the middle of the bridges adjustment area. If you get it in that area you can then adjust up or down to achieve the best compromise of being in tune. I say compromise because it truly is, its never perfect but you want it as good as you can make it obviously.
That's why most people don't like kits that have the bridge pre-positioned in a spot, it just is almost always not in the right place either up or down and back or forth. Good luck on your project!